af647 p21  (Cell Signaling Technology Inc)

Journal: Dose-Response

Article Title: Effects of Low Dose Neutron-Gamma Field on Cell Cycle and Damage of Human Lymphocytes

doi: 10.1177/15593258251323789

Figure Lengend Snippet: The expression levels of proteins related to the cell cycle exhibited dose-dependent variations in response to radiation. The expression changes of γH2AX, p21, ATM, p53 and CDK2 are depicted in pictures (A), (B), (C), (D), (E) respectively. The vertical axis in the given images represents the relative expression of cyclin, while the horizontal axis represents the radiation dose. The statistical method was employed to analyze the disparity between each radiation dose group and the “0” dose group. *, P < 0.05; **, P < 0.01, ***, P < 0.001.

Article Snippet: p21-mAb-AF647 , CST (US).

Techniques: Expressing

Journal: Dose-Response

Article Title: Effects of Low Dose Neutron-Gamma Field on Cell Cycle and Damage of Human Lymphocytes

doi: 10.1177/15593258251323789

Figure Lengend Snippet: The mRNA expression of p21 and CDK2 genes exhibited dose-dependent changes. The horizontal coordinate showed the irradiated dose, and the vertical coordinate showed the mRNA expression of p21,CDK2. The statistical method was employed to analyze the disparity between each radiation dose group and the “0” dose group, with the distinction being denoted by “*” (n = 3). *, P < 0.05; **, P < 0.01, ***, P < 0.001.

Article Snippet: p21-mAb-AF647 , CST (US).

Techniques: Expressing, Irradiation

Journal: Dose-Response

Article Title: Effects of Low Dose Neutron-Gamma Field on Cell Cycle and Damage of Human Lymphocytes

doi: 10.1177/15593258251323789

Figure Lengend Snippet:

Article Snippet: p21-mAb-AF647 , CST (US).

Techniques: RNA Extraction

Journal: bioRxiv

Article Title: RNA-binding proteins control the G2-M checkpoint of the germinal centre B cell

doi: 10.1101/2024.12.01.626220

Figure Lengend Snippet: B cells isolated from LNs of Zfp36l1 fl/fl Zfp36l2 fl/fl CD23 cre mice or Zfp36l1 fl/fl Zfp36l2 fl/fl control mice were co-cultured for 7 days in the presence of CD40LB-expressing feeder cells. a) BrdU and 7AAD staining of CD95 + CD138 − GC-like B cells. b) Venn diagram for genes within the Reactome “Cell cycle” gene set, showing the overlap between those that are increased in Zfp36l1 fl/fl Zfp36l2 fl/fl CD23 cre GC-like B cells compared to Zfp36l1 fl/fl Zfp36l2 fl/fl control cells, bound by mCherry-ZFP36L1 in their 3’UTR, or contain two UAUU motifs separated by up to three nucleotides within their 3’UTR. c) Left: schematic representation of cyclins and CDKs regulating cell cycle progression; iCLIP targets in yellow. Right: Log2 fold change (KO/control) of mRNA encoding cyclins and CDKs as measured by RNA-seq. In yellow iCLIP targets encompassing UAUUN{0-3}UAUU motif within their 3’UTR; in grey mRNAs containing AU-rich binding motifs but not identified as iCLIP targets; in black mRNA without UAUUN{0-3}UAUU binding motif. d, e, g) iCLIP data from GC-like B cells (d7) showing reads across Ccnb1 (d) , Cdk1 (e) , Cdkn1a (g) transcripts with an expanded view of the 3’UTR. f) Histograms depict protein expression of indicated cell-cycle related genes. n= 3 mice per group; data representative of at least two independently performed experiments. h) Left: graph displays normalized counts (mean±SD) of Cdkn1a as measured by RNAseq. Right: protein expression as measured by flow cytometry. n= 3 mice per group.

Article Snippet: For replication stress studies, intracellular staining was carried out with the following rabbit monoclonal antibodies: anti-p53-AF647 (1C12 - Cell Signalling), anti-p21-AF647 (F5 - Santa Cruz), or unconjugated rabbit monoclonal anti-pATM (Ser1981, D25E5 - Cell Signalling), anti-cyclin D2 (D52F9 - Cell Signalling), anti-cyclin E2 (E142-Abcam), anti-cyclin B1 (D5C10 - Cell Signalling), anti-pCdc2 (Tyr15; 10A11 - Cell Signalling), anti-CDK2 (78B2 - Cell Signalling), anti-pCHK1 (Ser345; 133D3 - Cell Signalling), or IgG isotype control (Da1E - Cell Signalling); and the following rabbit polyclonal antibodies: anti-pATR (Ser428) and anti-Cdc2 (CDK1; both Cell Signalling).

Techniques: Isolation, Control, Cell Culture, Expressing, Staining, RNA Sequencing, Binding Assay, Flow Cytometry